Virus:Article Title: Inhibition of MAPK p38α overcomes the cancer immunosurveillance defect caused by FPR1 loss-of-function mutation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies αβ-actin (HRP) Biolegend Cat# 664804 αERK1/2 Biolegend Cat# 686902 αERK1/2 Phospho (Thr202/Tyr204) Biolegend Cat# 369502 αMKK3 Phospho (Ser189/Ser207) Cell Signaling Technology Cat# 12280 αMAPKAPK2 (MK2) Life Technologies MA551342 αMAPKAPK2 Phospho (Thr334) Life Technologies MA528081 αPD-1(blockade) BioXcell Cat# BE0273 Rat IgG2a BioXcell Cat# BE0089 αIL1β Biolegend Cat# 503502 Biotin-conjugated αIL1β Biolegend Cat# 515801 αIL2 Biolegend Cat# 503702 Biotin-conjugated αIL2 Biolegend Cat# 503804 αIL12(p70) Biolegend Cat# 511802 Biotin-conjugated αIL12(p70) Biolegend Cat# 505302 αTNFα Biolegend Cat# 506102 Biotin-conjugated αTNFα Biolegend Cat# 516003 αCD3_BUV737 BD Bioscience Cat# 612803 αCD4_BUV496 BD Bioscience Cat# 612952 αFoxp3_FITC BD Bioscience Cat# 567453 αIL17A_APC/Cy7 BD Bioscience Cat# 560821 αCD3_PerCP/Cy5.5 Life Technologies Cat# 45-0031-82 αCD8_PerCP/Cy5.5 Life Technologies Cat# 45-0081-82 αCD44_APC Life Technologies Cat# 17-0441-82 αCD62L Alexa Fluor 700 Life Technologies Cat# 56-0621-82 αCD69_eFluor 450 Life Technologies Cat# 48-0691-82 αGranzyme B-FITC Life Technologies Cat# 11-8898-82 αIL2_PE/Cy7 Life Technologies Cat# 25-7021-82 αIFNγ_PE Life Technologies Cat# 12-7311-82 αTNFα_APC Life Technologies Cat# 17-7321-82 αKi-67_eFluor 450 Life Technologies Cat# 48-5698-82 αKi-67-BV650 Life Technologies Cat# 416-5698-82 αCD45_BV786 Life Technologies Cat# 417-0451-82 αCD8_APC/FireTM 750 Biolegend Cat# 100766 αCD25_BV650 Biolegend Cat# 102038 αCD45_Alexa Fluor 700 Biolegend Cat# 103128 αCD45_APC Biolegend Cat# 103112 αCD45.1_PE/Cy7 Cat# 110730 αICOS_PE Cat# 117406 αCTLA4_PE/Cy7 Cat# 106314 αPD-1_BV786 Cat# 135225 Bacterial and virus strains Edit-R lentiviral CAG-Blast-Cas9 nuclease particles Horizon Discovery Cat# VCAS10129 pCDH-lenti-FPR1_WT This paper N/A (Continued on next page) Cell Reports Medicine 7, 102683, March 17, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCDH-lenti-FPR1_T360E This paper N/A pCDH-lenti-Empty vector This paper N/A pCDH-lenti-ZsGreen-OVA This paper N/A Biological samples Healthy adult peripheral blood mononuclear cells (PBMCs) CTIBiotech France Cat# CTICC.IMPB.1 Chemicals, peptides, and recombinant proteins β-mercaptoethanol Sigma-Aldrich Cat# M3148 Dexamethasone Sigma-Aldrich Cat# D0700000 Doxycycline hyclate Sigma-Aldrich Cat# D3000000 Recombinant murine GM-CSF Miltenyi Biotec Cat# 130-095-735 SCREEN-WELL® ICCB Known Bioactives library Enzo Life Sciences Cat# BML-2840 MAPK Compound Library MedChemExpress Cat# HY-L010; library ID: HY-LD-000005319 SB203580 MedChemExpress Cat# HY-10256 SB239063 MedChemExpress Cat# HY-11068 Skepinone-L MedChemExpress Cat# HY-15300 Pamapimod MedChemExpress Cat# HY-10405 Doramapimod MedChemExpress Cat# HY-10320 Ralimetinib MedChemExpress Cat# HY-13241 Losmapimod MedChemExpress Cat# HY-10402 PD98059 MedChemExpress Cat# HY-12028 Cyclosporin H MedChemExpress Cat# HY-P1122 HCH6-1 MedChemExpress Cat# HY-101283 Bumetanide MedChemExpress Cat# HY-17468 DDR1-IN-1 MedChemExpress Cat# HY-13979 DDR1-IN-2 MedChemExpress Cat# HY-U00444 N-Formyl-Met-Leu-Phe (FMLP) Sigma-Aldrich Cat# 47729-10MG-F ERK Activation Inhibitor Peptide I, Cell-Permeable Sigma-Aldrich Cat# 328000 Ovalbumin (OVA) Sigma-Aldrich Cat# A5503 Oxaliplatin (OXA) Sigma-Aldrich Cat# Y0000271 Mitoxantrone dihydrochloride (MTX) Sigma-Aldrich Cat# M6545 cyclophosphamide (CTX) Sigma-Aldrich Cat# C3250000 Azoxymethane (AOM) Sigma-Aldrich Cat# A5486 dextran sulfate sodium (DSS) Life Technologies Cat# J63606.22 CFSE Biolegend Cat# 423801 Collagenase type IV Life Technologies Cat# 17104019 DNAse I Sigma-Aldrich Cat# DN25 D-Biotin Sigma-Aldrich Cat# B4639 Recombinant human GM-CSF Miltenyi Biotec Cat# 130-093-867 Recombinant Human IFN-α2 Biolegend Cat# 592706 Human Sera Type AB, Heat-inactivated Institut de Biotechnologies Jacques Boy Cat# 201021334 Critical commercial assays PKH26 staining kit Sigma-Aldrich Cat# PKH26GL DharmaFECT #1 transfection reagent Horizon Discovery Cat# T-2001-04 DharmaFECT Duo transfection reagent Horizon Discovery Cat# T-2010-03 MycoStripTM Mycoplasma Detection Kit InvivoGen Cat# rep-mysnc-100 CellTracker blue Life Technologies Cat# C2110 CellTracker red Life Technologies Cat# C34552 (Continued on next page) e2 Cell Reports Medicine 7, 102683, March 17, 2026
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